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Dual effects of glucose on the cytosolic Ca 2+ activity of mouse pancreatic β‐cells
Author(s) -
Rorsman P.,
Abrahamsson H.,
Gylfe E.,
Hellman Bo
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81398-8
Subject(s) - cytosol , extracellular , intracellular , chemistry , stimulation , biophysics , basal (medicine) , calcium , channel blocker , biochemistry , medicine , endocrinology , biology , enzyme , insulin , organic chemistry
The cytosolic Ca 2+ activity of mouse pancreatic β‐cells was studied with the intracellular fluorescent indicator quin2. When the extracellular Ca 2+ concentration was 1.20 mM, the basal cytosolic Ca 2+ activity was 162 ± 9 nM. Stimulation with 20 mM glucose increased this Ca 2+ activity by 40%. In the presence of only 0.20 mM Ca 2+ or after the addition of the voltage‐dependent Ca 2+ ‐channel blocker D‐600, glucose had an opposite and more prompt effect in reducing cytosolic Ca 2+ by about 15%. It is concluded that an early result of glucose exposure is a lowering of the cytosolic Ca 2+ activity and that this effect tends to be masked by a subsequent increase of the Ca 2+ activity due to influx of Ca 2+ through the voltage‐dependent Ca 2+ channels.