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Molecular mechanism of uncoupling in brown adipose tissue mitochondria
Author(s) -
Kopecký Jan,
Guerrieri Ferruccio,
Ježek Petr,
Drahota Zdeněk,
Houštěk Josef
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81396-4
Subject(s) - mitochondrion , chemistry , valinomycin , biophysics , brown adipose tissue , adipose tissue , potassium , permeability (electromagnetism) , membrane potential , swelling , membrane , inner mitochondrial membrane , biochemistry , biology , organic chemistry , chemical engineering , engineering
Specific permeability properties of the inner membrane of brown adipose tissue mitochondria were analysed with the aid of simultaneous pH measurements outside mitochondria and of mitochondria swelling. It was shown that valinomycin‐induced potassium diffusion potential drives a parallel passive uptake of chlorides and extrusion of proton. Electrogenic H + ‐extrusion was independent on anion transport, no competition was found between the two processes and the former process exerted a lower sensitivity to the inhibitory effect of GDP. The existence of two distinct, independent pathways for translocation of protons and halide anions across the membrane is suggested.