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Reconstitution of ornithine transport in liposomes with Lubrol extracts of mitochondria
Author(s) -
Hommes F.A.,
Eller A.G.,
Evans B.A.,
Carter A.L.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81384-8
Subject(s) - sonication , liposome , ornithine , mitochondrion , chemistry , biochemistry , kinetics , phospholipid , vesicle , chromatography , membrane , amino acid , physics , arginine , quantum mechanics
The ornithine translocase of beef liver mithochondria was extracted with Lubrol WX and reconstituted in liposomes. The uptake of ornithine by the reconstituted vesicles followed Michaelis‐Menten kinetics, and was dependent on the intraliposomal pH, the time of sonication of the reconstituted liposomes and the phospholipid to detergent ratio. It is concluded that the ornithine translocator can be reconstituted, which makes the purification of this translocator feasible.