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Molecular cloning and restriction mapping of a Simian virus 40 deletion mutant derived from Simian transformants expressing a non‐karyophilic T antigen
Author(s) -
Fantuzzi Lia Fischer,
Vesco Cesare
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81383-6
Subject(s) - microbiology and biotechnology , simian , biology , molecular cloning , mutant , dna , sv40 large t antigen , transfection , restriction map , virus , restriction fragment , virology , gene , plasmid , genetics , complementary dna
A replication‐defective Simian virus 40 genome, with a deletion of about 120 nucleotides in the region encoding the N‐terminal fourth of the large T antigen, has been isolated from the DNA of Simian cells transformed by SV40. Both the original transformation, and the murine transformants obtained by transfection with this cloned mutant DNA, produced a large T antigen displaying in immunofluorescence an exclusively cytoplasmic localization. The protein apparent molecular mass (83 kDa) was about 6% smaller than that of normal karyophilic large T. Restriction analysis showed that the deletion eliminated two close Hin fI sites, at nucleotides 4459 and 4376 (map unit 0.50).