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Solubilization in high yield of opioid receptors retaining high‐affinity delta, mu and kappa binding sites
Author(s) -
Demoliou-Mason Catherine D.,
Barnard Eric A.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81348-4
Subject(s) - kappa , receptor , chemistry , yield (engineering) , solubilization , delta , κ opioid receptor , opioid , stereochemistry , biochemistry , mathematics , physics , thermodynamics , geometry , astronomy
The binding sites for opiates (agonist and antagonist) and opioid peptides can be solubilized from rat brain membranes with digitonin in the presence of Mg 2+ (10 mM). High affinity and high capacity binding to the soluble δ, μ, and κ receptors is obtainable when the membranes are treated in Mg 2+ (30°C, 60 min) prior to solubilization. The yields of solubilized binding sites extracted with digitonin, 40–90%, are higher than those obtained from Mg 2+ ‐pretreated membranes with other detergents commonly used for receptor solubilization. The stability of the digitonin‐soluble opioid receptor at room temperature makes it useful for purification and characterization.