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Role of the 33‐kDa polypeptide in preserving Mn in the photosynthetic oxygen‐evolution system and its replacement by chloride ions
Author(s) -
Miyao Mitsue,
Murata Norio
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81342-3
Subject(s) - oxygen evolution , photosystem ii , chemistry , oxygen , urea , spinach , photosynthesis , chloroplast , chloride , nuclear chemistry , oxygen evolving complex , manganese , inorganic chemistry , biochemistry , crystallography , electrochemistry , organic chemistry , electrode , gene
Treatment of Photosystem II particles from spinach chloroplasts with Triton X‐100 with 2.6 M urea in the presence of 200 mM NaCl removed 3 polypeptides of 33 kDa, 24 kDa and 18 kDa, but left Mn bound to the particles. The (urea + NaCl)‐treated particles could evolve oxygen in 200 mM, but not in 10 mM NaCl. Mn was gradually released with concomitant loss of oxygen‐evolution activity in 10 mM NaCl but not in 200 mM Cl − . The NaCl‐treated particles, which contained Mn and the 33‐kDa polypeptide but not the 24‐kDa and 18‐kDa polypeptides, did not lose Mn or oxygen‐evolution activity in 10 mM NaCl. These observations suggest that the 33‐kDa polypeptide maintains the binding of Mn to the oxygen‐evolution system and can be functionally replaced by 200 mM Cl − .