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Primary structure of the major glycans of the N ‐acetyllactosamine type derived from the human immunoglobulins M from two patients with Waldenström's macroglobulinemia
Author(s) -
Cahour A.,
Debeire P.,
Hartmann L.,
Montreuil J.,
Van Halbeek H.,
Vliegenthart J.F.G.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81341-1
Subject(s) - macroglobulinemia , waldenstrom macroglobulinemia , chemistry , glycan , antibody , concanavalin a , carbohydrate , biochemistry , glycoprotein , immunology , medicine , in vitro , lymphoma , multiple myeloma
The carbohydrate chains of the pathological human immunoglobulins M from two patients with Waldenström's macroglobulinemia were released by hydrazinolysis. The N ‐acetyllactosamine‐type glycans were obtained by affinity chromatography on concanavalin A and fractionated by high‐voltage paper electrophoresis. The primary structure of the major compounds was elucidated on the basis of carbohydrate analysis, methylation analysis, including mass‐spectrometry, and 500 MHz 1 H‐NMR spectroscopy. For both patients, this appeared to be a monosialyl monofucosyl biantennary structure; the compounds differed by the presence of an intersecting N ‐acetylglucosamine residue.