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Electron microscopic investigations on the structure of lectin I from Viscum album L.
Author(s) -
Lutsch G.,
Noll F.,
Ziska P.,
Kindt A.,
Franz H.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81339-3
Subject(s) - viscum album , lectin , uranyl acetate , monomer , substructure , uranyl , negative stain , crystallography , molecule , chemistry , staining , electron microscope , biophysics , materials science , ion , biology , biochemistry , physics , organic chemistry , optics , ecology , genetics , structural engineering , engineering , polymer
Molecules of mistletoe lectin I (ML I) were investigated electron microscopically by negative staining with uranyl salts. An equilibrium was found between monomers and dimers at the used protein concentration. ML I monomers show a nearly triangular or rounded profile with dimensions of 80 × 90 Å. ML I dimers are rod‐like particles composed of two triangular parts which are dislocated against each other. In another view the dimers appear thinner without a pronounced substructure. The dimensions of the dimers are 175 × 80 × 60 Å.