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Double‐quantum 1 H NMR studies of nitrogen metabolism in yeast
Author(s) -
Paul Juretschke Hans
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81254-5
Subject(s) - yeast , chemistry , nitrogen , nuclear magnetic resonance spectroscopy , proton , proton nmr , ammonium sulfate , nmr spectra database , nuclear magnetic resonance , analytical chemistry (journal) , spectral line , biochemistry , stereochemistry , chromatography , physics , organic chemistry , quantum mechanics , astronomy
Using double‐quantum 1 H NMR spectroscopy, the nitrogen metabolism of yeast cells pulse‐labelled with 15 N‐enriched ammonium sulfate has been detected. The sensitivity of this indirect method is far higher than direct observation of the 15 N signals by 15 N NMR. Hence, a spectrum with an excellent signal‐to‐noise ratio was obtained in 25.6 s. The observed proton signals were assigned to specific metabolites using two‐dimensional reverse shift correlation spectra.