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Isolation and structure of the bacterial sex pheromone, cAD1, that induces plasmid transfer in Streptococcus faecalis
Author(s) -
Mori Masaaki,
Sakagami Youji,
Narita Michihiko,
Isogai Akira,
Fujino Masahiko,
Kitada Chieko,
Craig Ronald A.,
Clewell Don B.,
Suzuki Akinori
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81248-x
Subject(s) - pheromone , plasmid , sex pheromone , chemistry , isolation (microbiology) , streptococcus , biochemistry , microbiology and biotechnology , biology , bacteria , dna , genetics
The Streptococcus faecalis sex pheromone cAD1, which is involved in the conjugative transfer of the hemolysin plasmid pAD1, has been isolated and its structure determined. Its M r is 818 and its amino acid sequence is H‐Leu‐Phe‐Ser‐Leu‐Val‐Leu‐Ala‐Gly‐OH. A replicate of the pheromone synthesized by the liquid phase method showed the same biological activity and Chromatographie behavior as the isolated cAD1. Pheromone activity was detectable at a concentration of ~ 5 × 10 −11 M.