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Intermediates in the conversion of prekeratin into keratin molecules in human epidermis
Author(s) -
Katagata Yohtaro,
Aso Kazuo
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81247-8
Subject(s) - keratin , epidermis (zoology) , chemistry , molecule , in vitro , biochemistry , microbiology and biotechnology , biology , anatomy , genetics , organic chemistry
In order to elucidate the relationship between prekeratin and keratin, we performed pulse‐chase experiments using [ 35 S]methionine ( 35 S‐Met) in vitro. Of 6 prekeratin molecules (49, 52, 55, 62, 69 and 71 kDa) that incorporated 35 S‐Met, the 55‐kDa prekeratin incorporated the most 35 S‐Met. In 3 molecules (52, 55 and 62 kDa) incorporation was decreased at 30 min after being chased; however, incorporation of only two molecules (55 and 62 kDa) of the 6 prekeratins was increased at 60 min. From these results and our previous data, we conclude that the initial stage of processing is as follows: 3 prekeratin molecules (52, 55 and 62 kDa) are first cleaved in the N‐terminal region, then two prekeratin molecules (55 and 69 kDa) are processed to intermediates (52 and 62 kDa) by some proteolytic enzyme(s).