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In vitro enzymatic methylation of DNA substituted by N ‐2‐aminofluorene
Author(s) -
Pfohl-Leszkowicz A.,
Fuchs R.P.P.,
Dirheimer G.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81240-5
Subject(s) - in vitro , dna methylation , chemistry , dna , methylation , enzyme , biochemistry , microbiology and biotechnology , biology , gene expression , gene
Both the initial velocity and the overall methylation of DNA substituted by aminofluorene, by a rat liver DNA(cytosine‐5‐)‐methyltransferase, are increased as compared to native DNA. The K m and V max of the modified DNA for the enzyme increase as a function of the extent of modification. The carcinogen may induce a secondary structure favouring the ‘walking’ of the enzyme along the DNA. The hypermethylation caused by this carcinogen could have a significance in gene activity, cellular differentiation and cancer induction.