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Chemiluminescent assay of lipid peroxide in plasma using cytochrome c heme peptide
Author(s) -
Iwaoka Teiki,
Tabata Fumiko
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81237-5
Subject(s) - chemiluminescence , chemistry , heme , hemeprotein , lipid peroxidation , peroxide , lipid peroxide , peptide , cytochrome c , biochemistry , luminescent measurements , hemoglobin , luminescence , photochemistry , chromatography , organic chemistry , enzyme , mitochondrion , optoelectronics , physics
A convenient assay specific to lipid hydroperoxide in plasma is presented. Cytochrome c heme peptide obtained from Saccharomyces was found to emit a strong chemiluminescence with any hydroperoxide, but not with TBA‐reactive substances. The benefit of measuring this luminescence using photon counting is discussed with respect to in vivo lipid peroxidation.