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Glycogen metabolism: a 13 C‐NMR study on the isolated perfused rat heart
Author(s) -
Lavanchy N.,
Martin J.,
Rossi A.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81234-x
Subject(s) - glycogen , perfusion , isoprenaline , chemistry , nuclear magnetic resonance spectroscopy , metabolism , medicine , endocrinology , biochemistry , biology , stereochemistry , stimulation
Glycogen synthesis from D‐[1‐ 13 C]glucose was observed in the perfused rat heart by 13 C‐NMR spectroscopy at 62.9 MHz. The glycogenogenesis was stimulated by pretreatment of the animals with isoprenaline. Whereas in hearts from control rats the incorporation of D‐[1‐ 13 C]glucose into the glycogen remained below the detection threshold, 5 min proton‐decoupled 13 C‐NMR spectra revealed, in hearts from treated rats, a significant labelling of the glycogen within the first minutes of the perfusion and a further linear increase of the glycogen resonance for up to 25 min. This model was used to monitor the appearance of 13 C‐labelled lactate during ischemia.