Premium
Incorporation of chromaffin granule membranes into large‐size vesicles suitable for patch‐clamp recording
Author(s) -
Picaud Serge,
Marty Alain,
Trautmann Alain,
Grynszpan-Winograd Odile,
Henry Jean-Pierre
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81231-4
Subject(s) - granule (geology) , vesicle , patch clamp , membrane , biophysics , chemistry , centrifugation , fluorescein , chromatography , liposome , incubation , fluorescence , materials science , biochemistry , biology , receptor , physics , quantum mechanics , composite material
Incubation of chromaffin granules with excess liposomes at pH 6.0 resulted in the formation of cell‐size structures, which were purified by centrifugation on sucrose gradients. Experiments with fluorescein‐labeled granules indicated incorporation of granule membrane to these structures. The preparation contained various vesicular structures with a diameter up to 15 μm. The largest elements were studied by the ‘patch‐clamp’ technique. ‘Cell‐attached’ and ‘whole‐cell’ recordings indicated the presence of currents corresponding to unitary conductances ranging from 100 to 500 pS.