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A specific translocator for oxaloacetate transport in chloroplasts
Author(s) -
Hatch M.D.,
Dröscher L.,
Flügge U.I.,
Heldt H.W.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81230-2
Subject(s) - citrate synthase , chloroplast , spinach , photosynthesis , malate dehydrogenase , biochemistry , chenopodiaceae , chemistry , chloroplast membrane , thylakoid , enzyme , gene
Evidence is provided that oxaloacetate is taken up by chloroplasts from maize and spinach leaves by a high‐affinity carrier. Apparent K m values for oxaloacetate are about 45 and 9 μM, respectively. Uptake was measured by following [4‐ 14 C]oxaloacetate reduction in illuminated chloroplasts. With maize chloroplasts the rates of uptake were sufficient to account for photosynthesis. This carrier had a very low affinity for malate and 2‐oxoglutarate. Ratios of the K i , for malate or 2‐oxoglutarate to the K m for oxaloacetate were about 150 and 50, respectively.