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[ 3 H]Ouabain binding and 86 rubidium uptake during the dimethyl sulfoxide‐induced differentiation of human promyelocytic leukemia HL‐60 cells
Author(s) -
Ladoux A.,
Geny B.,
Marrec N.,
Abita J.P.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81220-x
Subject(s) - dimethyl sulfoxide , chemistry , rubidium , ouabain , leukemia , acute promyelocytic leukemia , microbiology and biotechnology , biochemistry , biology , sodium , immunology , organic chemistry , potassium , retinoic acid , gene
When HL‐60 cells were induced to differentiate into granulocyte‐like cells by culture in the presence of 1.3% dimethyl sulfoxide, an increase in the rate of ouabain‐sensitive 86 Rb transport was observed during the first 6 h followed by a constant decrease which became stable on day 4 at about 40% of control level. By contrast, the number of ouabain binding sites remained constant during the first 24 h then decreased with the same kinetics as that of the 86 Rb transport rate. These results suggest that alterations in ionic fluxes, through activation of the sodium pump, are part of the early events resulting in HL‐60 cell differentiation.