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Distinct effects of acetylcholine and glucose on 45 calcium and 86 rubidium efflux from mouse pancreatic islets
Author(s) -
Nenquin M.,
Awouters P.,
Mathot F.,
Henquin J.C.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81218-1
Subject(s) - acetylcholine , efflux , islet , extracellular , phospholipid , chemistry , pancreatic islets , calcium , endocrinology , medicine , metabolism , rubidium , insulin , carbohydrate metabolism , biochemistry , biology , potassium , membrane , organic chemistry
The similarities between the effects of acetylcholine and glucose on phospholipid metabolism in pancreatic islet cells prompted the comparison of their effects on ionic fluxes. Acetylcholine (1 μM) consistently increased 45 Ca 2+ efflux from mouse islets, whereas glucose increased it in the presence, but decreased it in the absence of extracellular Ca 2+ . Acetylcholine consistently accelerated 86 Rb + efflux, and this effect was augmented by Ca 2+ omission. On the other hand, glucose markedly inhibited 86 Rb + efflux, except when its concentration was raised from 10 to 15 mM in the presence of Ca 2+ . Unlike their effects on phospholipid metabolism, the ionic effects of the two insulin‐secretagogues are thus very different.