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NMR studies on oligo‐deoxyribonucleotides containing N 6 ‐methyladenine
Author(s) -
Fazakerley G.Victor,
Téoule Robert,
Guy André,
Guschlbauer Wilhelm
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81216-8
Subject(s) - deoxyribonucleotides , chemistry , dna , base pair , nucleotide , double stranded , stereochemistry , crystallography , deoxyribonucleotide , z dna , nuclear magnetic resonance spectroscopy , oligonucleotide , biochemistry , gene
500 MHz 1 H‐NMR and NOE measurements of d(GGATCC) and d(GGm 6 ATCC) show that both oligo‐nucleotides assume a B‐DNA conformation at low temperature. Around the melting temperature, however, the single and double strands of the N ‐methylated form are in slow exchange on the NMR time scale. The preferred conformation of the adenine methyl group, cis to N 1 , retards base pairing and also destabilizes the double helix.