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The effect of phallotoxins on the structure of F‐actin in myosin‐free ghost muscle fibres of rabbit
Author(s) -
Borovikov Yu.S.,
Bukatina A.E.,
Son'kir B.Ja.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81214-4
Subject(s) - phalloidin , actin , myosin , biophysics , biology , dimethyl sulfoxide , biochemistry , chemistry , cytoskeleton , cell , organic chemistry
Using polarized UV fluorescent microscopy it has been shown that phallotoxins (phalloidin‐sulfone, phalloidin‐sulfoxide‐B, phalloidin‐sulfoxide‐A and dithio‐phalloidin) cause an increase in tryptophan fluorescence anisotropy of F‐actin myofilaments in myosin‐free ghost muscle fibres of rabbit. The results obtained are considered to be evidence of conformational changes in F‐actin, induced by phallotoxins. These changes are irreversible to a significant extent, which points to a high degree of actin binding to both toxic and nontoxic phallotoxins.