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DNA topoisomerase activities in concanavalin A‐stimulated lymphocytes
Author(s) -
Taudou Georges,
Mirambeau Gilles,
Lavenot Catherine,
der Garabedian Arsène,
Vermeersch Jacqueline,
Duguet Michel
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81212-0
Subject(s) - topoisomerase , concanavalin a , dna , microbiology and biotechnology , dna replication , biology , dna synthesis , biochemistry , chemistry , in vitro
Topoisomerase activities have been measured in nuclear extracts of concanavalin A‐stimulated lymphocytes. In parallel with the wave of DNA synthesis, type II topoisomerase activity was considerably increased. After 72 h treatment, this activity was stimulated approx. 20‐fold over the activity in untreated cells. In contrast, type I topoisomerase was poorly stimulated after 24 h treatment, and 4‐5‐fold after 72 h. These findings, together with our previous results on regenerating rat liver, suggest a major role of topoisomerase II in DNA replication.