Premium
Sequence preferences in the binding to DNA of triostin A and TANDEM as reported by DNase I footprinting
Author(s) -
Low C.M.Loretta,
Olsei Richard K.,
Waring Michael J.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81209-0
Subject(s) - footprinting , deoxyribonuclease i , dna footprinting , dna , sequence (biology) , chemistry , tandem repeat , dna binding protein , genetics , biology , computational biology , microbiology and biotechnology , biochemistry , base sequence , gene , transcription factor , genome
Six or seven triostin‐binding sites have been identified in a 160‐base‐pair DNA restriction fragment containing the tyr T promoter sequence. Each is centred round a CpG step, and the minimum binding site‐size appears to be six base pairs. The sites are practically the same as those reported for echinomycin by DNase I digestion. Only two sites are protected by binding of TANDEM, the des‐ N ‐tetramethyl analogue of trios‐tin A; they are centred around the sequences ATA or TAT.