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Evidence for similar structural changes on binding of platinum anti‐tumor agents to DNA and nucleosomes
Author(s) -
Marzilli Luigi G.,
Reily Michael D.,
Heyl Barbara L.,
McMurray Cynthia T.,
Wilson W.David
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81203-x
Subject(s) - dna , polynucleotide , chemistry , nucleosome , conformational change , structural change , polymer , stereochemistry , biophysics , crystallography , histone , biochemistry , biology , organic chemistry , economics , macroeconomics
The influence of Pt(II) compounds on the 31 P NMR spectra of natural DNA, synthetic polynucleotides, and nucleosomes was investigated. With Pt complexes which are anti‐tumor agents, a new peak or shoulder centered at ~ 1.2 ppm downfield from the untreated DNA signal was observed. When Pt compounds known not to be anti‐tumor agents were studied, no such new signal was observed. The most reasonable explanation for the downfield resonance is that it is a consequence of a structural change induced in the DNA by the anti‐tumor agent. Since the effect of the Pt compounds on nucleosomes was similar, the same structural change is probably occurring in DNA in solution and in nucleosomes. A nonalternating dG · dC polymer, but not alternating G·C or any A·T polymers, exhibited a similar spectral change and this finding suggests that the structural change in the DNA arises primarily from reaction of Pt anti‐tumor agents with adjacent G residues.