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Malonyl CoA inhibition of carnitine palmityltransferase in rat heart mitochondria
Author(s) -
Paulson Dennis J.,
Ward Karen M.,
Shug Austin L.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81201-6
Subject(s) - carnitine , malonyl coa , mitochondrion , adenine nucleotide translocator , respiration , beta oxidation , carnitine o palmitoyltransferase , palmitoylcarnitine , biochemistry , chemistry , adenine nucleotide , fatty acid , biology , nucleotide , botany , gene
The effects ofmalonyl CoA on carnitine palmityltransferase I (CPT‐I), fatty acid‐supported state 3 respiration, and carnitine reversal of palmityl CoA inhibition of state 3 respiration and of the adenine nucleotide translocator, were studied in isolated rat heart mitochondria. Malonyl CoA was a potent competitive inhibitor of CPT‐I with an I 50 of 0.8 μM. Fasting did not affect CPT‐I activity or the I 50 value of malonyl CoA. Malonyl CoA inhibited fatty acid‐supported respiration and prevented carnitine from reversing the inhibition of the adenine nucleotide translocator by palmityl CoA. These findings suggest that malonyl CoA may affect fatty acid oxidation in the heart.