Premium
4‐( N‐tert ‐Butyloxycarbonylaminomethyl)‐phenylisothiocyanate: its synthesis and use in microsequencing
Author(s) -
Palacz Zbigniew,
Salnikow Johann,
Ju Shan-Wei,
Wittmann-Liebold Brigitte
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81198-9
Subject(s) - reagent , edman degradation , derivative (finance) , chemistry , chromatography , combinatorial chemistry , organic chemistry , biochemistry , peptide sequence , economics , financial economics , gene
The chemical synthesis of 4‐( N ‐ tert butyloxycarbonylaminomethyl)‐phenylisothiocyanate starting from 4‐nitrobenzylamine is described. This derivative represents an Edman‐type reagent with a masked amino group which renders the thiohydantoin upon deblocking susceptible to fluorogenic detection. The coupling efficiency is determined in comparison to degradations with PITC, DABITC and FITC. The detection sensitivity on thin layer chromatograms is compared to the thiohydantoins derived from DABITC.