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Complete separation and quantitative determination of DABTH‐amino acid derivatives by isocratic reversed‐phase high performance liquid chromatography
Author(s) -
Lehmann Arnold,
Wittmann-Liebold Brigitte
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81197-7
Subject(s) - chromatography , chemistry , high performance liquid chromatography , isoleucine , solvent , leucine , phenyl isothiocyanate , phase (matter) , reversed phase chromatography , detection limit , isothiocyanate , quantitative analysis (chemistry) , amino acid , degradation (telecommunications) , chromatographic separation , organic chemistry , biochemistry , telecommunications , computer science
The isocratic separation by reversed‐phase HPLC of all DABTH‐amino acid derivatives which are released by degradation of polypeptides with 4‐ N , N ‐dimethylaminobenzene‐4'‐isothiocyanate is described. The method allows an easy and quick identification and the quantitative determination of all derivatives in 30 min and includes the separation of isoleucine and leucine. Detection limit is 5 pmol at 436 nm. The solvent mixture is continuously recycled through the column, thereby reducing the solvent consumption considerably.