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A new approach for using cofactor dependent enzymes: example of alcohol dehydrogenase
Author(s) -
Sicsic Sames,
Durand Philippe,
Langrene Sylvie,
le Goffic François
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81188-6
Subject(s) - cofactor , nad+ kinase , alcohol dehydrogenase , chemistry , enzyme , nicotinamide , ethanol , alcohol oxidoreductase , biochemistry , substrate (aquarium) , alcohol , cyclohexanone , nicotinamide adenine dinucleotide , dehydrogenase , stereochemistry , catalysis , biology , ecology
The use of enzymes requiring a cofactor as substrate in organic synthesis is still a problem since the cofactors are expensive. This study deals with a new approach consisting of using fragments of NAD + . Three fragments of NAD(H) are examined. The activities of NMN + and NMNH are greatly improved by the addition of adenosine in ethanol oxidation and in cyclohexanone reduction, respectively. Nicotinamide monocucleoside is not active in the ethanol oxidation but the addition of AMP promotes this reaction.