Premium
Interaction between calmodulin and microtubule‐associated proteins prepared at different stages of brain development
Author(s) -
Erneux Christophe,
Passareiro Heloísa,
Nunez Jacques
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81148-5
Subject(s) - calmodulin , microtubule , trifluoperazine , microtubule associated protein , microbiology and biotechnology , tubulin , biology , chemistry , calmodulin binding proteins , biochemistry , biophysics , enzyme
Rat brain microtubules were prepared at the adult stage and from immature (i.e., 4‐day‐old) animals. At an early stage of development, the composition of microtubule‐associated proteins is qualitatively different from that found at the adult stage [(1982) Eur. J. Biochem. 129, 465‐471]. The influence of calmodulin on the time course of assembly of second cycle microtubules was compared at both stages of brain development (i.e., microtubules originating from 4‐day‐old and adult animals). In the presence of Ca 2+ the inhibition of microtubule assembly was more pronounced at a young stage of brain development than at the adult stage. Cross‐linking studies with 125 I‐labeled calmodulin further established that the two major microtubule‐associated proteins, MAP 2 and TAU were able to bind to calmodulin at both stages of brain development but with different intensities. The labeling with 125 I‐labeled calmodulin was Ca 2+ ‐dependent, specific, displaced by unlabeled calmodulin and trifluoperazine.