Premium
Characterization of new enkephalin‐containing peptides in the adrenal medulla by immunoblotting
Author(s) -
Patey Gilles,
Liston Dane,
Rossier Jean
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81146-1
Subject(s) - proenkephalin , adrenal medulla , enkephalin , radioimmunoassay , molecular mass , chemistry , medulla , peptide sequence , biochemistry , microbiology and biotechnology , biology , endocrinology , catecholamine , enzyme , gene , receptor , opioid
Immunoblotting combined with radioimmunoassays (RIAs) directed specifically towards certain sequences of the proenkephalin molecule has been used to characterize the enkephalin‐containing peptides (ECPs) present in the bovine adrenal medulla. Immunoblotting allowed the simultaneous visualization of all ECPs present in a crude extract of this gland. Combining this technique with RIAs we have been able to characterize a new high molecular mass ECP, a 23.3‐kDa protein which contains the amino‐terminal part of proenkephalin and ends with the sequence of Leu‐enkephalin at its carboxy‐terminus.