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Phospholipase A 2 activity of pancreatic secretory factor, a new secretagogue isolated from the venom of Heloderma suspectum
Author(s) -
Dehaye Jean-Paul,
Winand Jacques,
Michel Patrick,
Poloczek Piotr,
Damien Catherine,
Vandermeers-Piret Marie-Claire,
Vandermeers André,
Christophe Jean
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81142-4
Subject(s) - secretagogue , phospholipase a2 , calcium , phospholipase a , phospholipase , endocrinology , biology , medicine , venom , pancreas , secretion , biochemistry , enzyme
Pancreatic secretory factor (PSF), an efficient pancreatic secretagogue recently isolated from the venom of Heloderma suspectum is shown to exert phospholipase A 2 activity towards phosphatidylcholine. This activity is strictly dependent on calcium (apparent K a 40 nM) and has an optimum pH around 9. At pH 7.4 and in the presence of calcium, PSF retains 40% of its phospholipase A 2 activity. These results are compared to the calcium dependency of the secretory effect of PSF on rat pancreatic acini. Taken collectively, the present data on PSF suggest that a similar endogenous phospholipase A 2 activity might be involved in the late steps of stimulus‐secretion coupling in the exocrine pancreas.