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Intracellular pH measurements using the fluorescence of 9‐aminoacridine
Author(s) -
Vigne Paul,
Frelin Christian,
Lazdunski Michel
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81140-0
Subject(s) - nigericin , intracellular , intracellular ph , ionophore , fluorescence , chemistry , biophysics , membrane , biochemistry , biology , physics , quantum mechanics
9‐Aminoacridine is a fluorescent weak base which distributes across the plasma membrane according to the pH gradient, and can be used to monitor changes in intracellular pH in eukaryotic cells. A calibration technique using the ionophore nigericin is described which allows us to measure intracellular pH values of chick skeletal muscle cells.