Premium
Thrombin stimulates Na + ‐H + exchange across the human platelet plasma membrane
Author(s) -
Siffert W.,
Fox G.,
Mückenhoff K.,
Scheid P.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81139-4
Subject(s) - amiloride , thrombin , platelet , chemistry , choline , biophysics , ion transporter , membrane , intracellular , sodium , biochemistry , medicine , biology , organic chemistry
We have investigated the release of protons from thrombin‐stimulated platelets. Addition of thrombin to suspensions of washed platelets resulted in fast liberation of H + . In the presence of 0.1 mM amiloride, a potent inhibitor of the Na + /H + transport system, the amount of protons liberated was decreased by about 50%, and was further reduced to about 15% by 1 mM amiloride. Similar inhibition of H + release was observed after Na + in the incubating medium had been replaced by choline. We conclude that one of the earliest events in thrombin‐stimulated platelets consists of the activation of an Na + /H + countertransport, which leads to an increase in intracellular pH.