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Evidence for cytochrome bc 1 complex involvement in nitrite reduction in a photodenitrifier, Rhodopseudomonas sphaeroides forma sp. denitrificans
Author(s) -
Urata Katsuro,
Satoh Toshio
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81126-6
Subject(s) - cytochrome , nitrite , cytochrome c , chemistry , cytochrome c1 , nitrite reductase , cytochrome c peroxidase , coenzyme q – cytochrome c reductase , electron transport chain , paracoccus denitrificans , cytochrome b6f complex , biochemistry , cytochrome b , stereochemistry , enzyme , nitrate , organic chemistry , mitochondrion , mitochondrial dna , gene
The cytochrome bc 1 complex which has a quinol : cytochrome c 2 oxidoreductase activity was isolated from a photodenitrifier, Rhodopseudomonas sphaeroides forma sp. denitrificans . When duroquinol (tetramethyl‐ p ‐benzoquinol) was used as the electron donor, maximum nitrite reduction activity was obtained in an anaerobic mixture containing the isolated cytochrome bc 1 complex, nitrite reductase and cytochrome c 2 Nitrite reduction in chromatophore membranes, as well as in the reconstituted system, was inhibited in the presence of antimycin A. No nitrite reduction occurred in the absence of cytochrome c 2 . These results indicated that the cytochrome bc 1 complex involved in photosynthetic electron transfer also donates reducing equivalents to the dissimilatory nitrite reductase via cytochrome c 2 in this photodenitrifier.