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Immunological identification of four different polypeptides in ‘subunit VII’ of mammalian cytochrome c oxidase
Author(s) -
Kuhn-Nentwig Lucia,
Kadenbach B.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81123-0
Subject(s) - antiserum , cytochrome c oxidase , oxidase test , biochemistry , protein subunit , cytochrome , chemistry , microbiology and biotechnology , gel electrophoresis , enzyme , biology , antibody , immunology , gene
Rat liver cytochrome c oxidase was separated by SDS‐gel electrophoresis into 13 polypeptide bands. Monospecific antisera against the isolated polypeptides VIIa, VIIb and VIIc were raised in rabbits. Cytochrome c oxidase was blotted on nitrocellulose and incubated with the antisera. The antisera reacted only with their corresponding polypeptides, indicating no immunological relationship between polypeptides VIla, VIIb and VIIc. The data also exclude that these polypeptides are proteolytic breakdown products of larger subunits.