Premium
The isolation and sequencing of human gastric inhibitory peptide (GIP)
Author(s) -
Moody Alister J.,
Thim Lars,
Valverde Isabel
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81114-x
Subject(s) - edman degradation , gastric inhibitory polypeptide , peptide , peptide sequence , chemistry , biochemistry , amino acid , amino acid residue , sequence (biology) , microbiology and biotechnology , biology , gene , hormone , glucagon
Human GIP 1–42 and fragments of human GIP corresponding to GIP 10–42, GIP 11–42, and GIP 17–42 were isolated from acid‐ethanol extracts of human small intestines with the aid of an anti‐GIP serum specific for the extreme C‐terminal portion of the GIP molecule. The full sequence of human GIP has been established by Edman degradation of these peptides and fragments thereof by automatic gas‐phase sequencing. Human GIP differs from porcine GIP at residues 18 and 34. The sequence of human GIP is thus: Tyr‐Ala‐Glu‐Gly‐Thr‐Phe‐Ile‐Ser‐Asp‐Tyr‐Ser‐Ile‐Ala‐Met‐Asp‐Lys‐Ile‐His‐Gln‐Gln‐Asp‐Phe‐5 10 15 20 Val‐Asn‐Trp‐Leu‐Leu‐Ala‐Glu‐Lys‐Gly‐Lys‐Lys‐Asn‐Asp‐Trp,Lys‐His‐Asn‐Ile‐Thr‐Gln. Amino acid 25 30 35 40 residues 18 and 34 are Arg and Ser, respectively, in porcine GIP.