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Hydrocortisone inhibits prostaglandin production but not arachidonic acid release from cultured macrophages
Author(s) -
Wood John N.,
Coote Paul R.,
Rhodes John
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81093-5
Subject(s) - arachidonic acid , phospholipase a2 , prostaglandin , stimulation , chemistry , prostaglandin e2 , macrophage , phospholipase , cell culture , hydrocortisone , endocrinology , medicine , biology , biochemistry , in vitro , enzyme , genetics
We have investigated the action of hydrocortisosone on arachidonic acid mobilisation in cultures of mouse peritoneal macrophages, mouse L 929 cells and the mouse macrophage‐like cell line RAW 264 . Hydrocortisone inhibits both arachidonic acid release and prostaglandin production by L 929 cells. However, prostaglandin production by macrophages or RAW 264 cells is inhibited with a concomitant stimulation rather than inhibition of arachidonic acid release. These data suggest that hydrocortisone acts at the level of phospholipase activity in fibroblasts but at a later stage of prostanoid production in macrophages.