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1α,25‐Dihydroxyvitamin D 3 directly induces fusion of alveolar macrophages by a mechanism involving RNA and protein synthesis, but not DNA synthesis
Author(s) -
Tanaka Hirofumi,
Hayashi Takamune,
Shiina Yoshiko,
Miyaura Chisato,
Abe Etsuko,
Suda Tatsuo
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81078-9
Subject(s) - cycloheximide , protein biosynthesis , dna synthesis , rna , chemistry , microbiology and biotechnology , dna , biochemistry , biology , gene
The results of our present study indicate that 1α,25‐dihydroxyvitamin D 3 [1α,25(OH) 2 D 3 ] directly induces fusion of mouse alveolar macrophages without any participation of T‐lymphocytes by a mechanism involving RNA and protein synthesis but not DNA synthesis. We have reported that 1α,25(OH) 3 D 3 induces fusion of alveolar macrophages by a direct mechanism and by a spleen cell‐mediated indirect mechanism [(1983) Proc. Natl. Acad. Sci. USA 80, 5583‐5587]. Alveolar macrophages pretreated with or without anti‐Thy 1.2 antibody and complement fused similarly when they were incubated with 1α,25(OH) 2 D 3 . The vitamin suppressed DNA synthesis, but it significantly enhanced RNA and protein synthesis. The 1α,25(OH) 2 D 3 ‐induced fusion was blocked by adding actinomycin D or cycloheximide, but not by hydroxyurea.