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Structures of fucose‐containing ceramide pentasaccharides from the plasma of blood group O Le(a − b − ) nonsecretors
Author(s) -
Pfannschmidt Gerhard,
Peter-Katalinić Jasna,
Kordowicz Maria,
Egge Heinz,
Dabrowski Janusz,
Dabrowski Ursula,
Hanfland Peter
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81077-7
Subject(s) - glycosphingolipid , chemistry , ceramide , glycolipid , fucose , mass spectrometry , chromatography , beta (programming language) , biochemistry , microbiology and biotechnology , galactose , biology , apoptosis , computer science , programming language
A minor, Le c blood‐group inactive ceramide pentasaccharide double band fraction has been isolated from the plasma of blood group O Le(a − b − ) nonsecretors. The two purified glycolipids were analysed by NMR spectroscopy, mass spectrometry and combined gas chromatography‐mass spectrometry. The following structures could be established: GlcNAc(β1 → 3)Gal(β1 → 4)Glc(β1 → 1)Cer (I); Gal(β1 → 4) [Fuc(α1 → 3)]GlcNAc(β1 → 3)Gal(β1 → 4)Glc(β1 → 1)Cer (II). It must be concluded that at least part of the secretor gene‐independent plasmatic H type 2 blood‐group activity can be attributed to glyco‐sphingolipid I, whereas substance II, originally detected in cancerous tissue, also occurs in the plasma of healthy individuals.