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Subcellular localisation of cerebral fucosyltransferase
Author(s) -
Broquet Pierre,
Serres-Guillaumond Mireille,
Baubichon-Cortay Hélène,
Peschard Marie-Jo,
Louisot Pierre
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81074-1
Subject(s) - fucosyltransferase , fucose , microsome , subcellular localization , biochemistry , mitochondrion , ficoll , chemistry , cell fractionation , glycoprotein , biology , microbiology and biotechnology , enzyme , gene , in vitro , peripheral blood mononuclear cell
GDP‐fucose : asialofetuin fucosyltransferase from sheep brain was fractionated on a sucrose gradient into two activity peaks. Using purification on Ficoll adapted from the proposed method [(1980) J. Neurochem. 35, 281‐296], double localisation of cerebral fucosyltransferase was confirmed and the subcellular active fractions identified as light microsomes and mitochondria.