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Nucleotide sequence of the lspA gene, the structural gene for lipoprotein signal peptidase of Escherichia coli
Author(s) -
Yu Fujio,
Yamada Hisami,
Daishima Kyoko,
Mizushima Shoji
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81060-1
Subject(s) - gene , escherichia coli , nucleic acid sequence , sequence (biology) , biology , genetics , signal peptidase , peptide sequence , signal peptide , structural gene , nucleotide , microbiology and biotechnology
The nucleotide sequence of the lspA gene coding for lipoprotein signal peptidase of Escherichia coli was determined and the amino acid sequence of the peptidase was deduced from it. The molecular mass and amino acid composition of the predicted lipoprotein signal peptidase were consistent with those of the signal peptidase purified from cells harboring the lspA gene‐carrying plasmid. The peptidase most probably has no cleavable signal peptide. The lspA gene was preceded by the ileS gene coding for isoleucylt RNA synthetase and the tandem termination codons of the ileS gene overlapped with the initiation codon of the lspA gene.