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Increase in temperature induces the Z to B transition of poly[d(G‐C)] in water‐ethanol solution
Author(s) -
Karapetyan Armen T.,
Minyat Elvira E.,
Ivanov Valery I.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81055-8
Subject(s) - polynucleotide , enthalpy , circular dichroism , chemistry , ethidium bromide , ethanol , kinetics , transition temperature , crystallography , transition (genetics) , glass transition , thermodynamics , polymer , organic chemistry , biochemistry , dna , physics , superconductivity , quantum mechanics , gene
An increase in temperature from 20 to 50° C results in the complete transition from the Z to B form of poly(d(G‐C)], dissolved in a 55% ethanol‐water solution. The transition is fully reversible and displays a slow kinetics. The transition profiles for the free polynucleotide and for that in the presence of ethidium bromide, which is known to stabilize the B form, are obtained by circular dichroism. Based on these data the enthalpy value for the B‐Z transition in our conditions is estimated to be Δ H BZ = −0.7 .