Premium
In vitro methylation of total and foldback DNAs in normal and virus‐transformed cells
Author(s) -
Voipe Pietro,
Eremenko Tamilla
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81053-4
Subject(s) - methylation , in vitro , dna methylation , dna , biology , microbiology and biotechnology , palindrome , virus , cpg site , chemistry , virology , genetics , genome , gene , gene expression
The levels of the in vitro methylation of total and palindromic DNAs in nuclei isolated from normal and virus‐transformed cells are compared. The methylation rate of total DNA in normal rat kidney cells is much higher than that detected in normal mouse fibroblasts. However, for both cell species, while the maximal rate of DNA methylation is observed in the mid‐logarithmic phase of the cell culture growth, palindromes are always found to be more heavily methylated than total DNA. The 5‐methylcytosine content of DNA, especially of palindromes, is higher in virus‐transformed cells than in untransformed cells.