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Competitive solid‐phase immunoassay of testosterone using time‐resolved fluorescence
Author(s) -
Bertoft E.,
Eskola J.U.,
Näntö V.,
Lövgren T.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81049-2
Subject(s) - polyclonal antibodies , chemistry , immunoassay , bovine serum albumin , chromatography , conjugate , monoclonal antibody , detection limit , radioimmunoassay , serum albumin , antibody , biochemistry , biology , immunology , mathematical analysis , mathematics
A competitive solid‐phase immunoassay for the determination of testosterone in serum samples using time‐resolved fluorescence is described. The solid phase is a testosterone‐3‐( O ‐carboxymethyl)‐oxime‐ovalbumin conjugate coated to polystyrene microtiter strips. Europium‐labelled polyclonal and monoclonal antibodies against testosterone‐3‐( O ‐carboxymethyl)‐oxime‐bovine serum albumin were compared. Their behavior was quite similar although the polyclonal antibody was more sensitive, giving a detection limit of 15 fmol testosterone per assay. Correlation with RIA was very good ( r = 0.982 and y = −0.150 + 0.969 x ).