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Methylation of the SV40 Hpa II site does not affect late viral gene expression in microinjected tissue culture cells
Author(s) -
Graessmann M.,
Ziechmann C.,
Graessmann A.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81036-4
Subject(s) - microbiology and biotechnology , biology , dna methylation , dna replication , xenopus , methylation , dna , gene , enhancer , gene expression , genetics
Intranuclear coinjection of the late SV40 Kpn I/ Bcl l DNA fragment and the early promotor/enhancer Hpa II/ Bgl I DNA segment into permissive monkey and non‐permissive mouse cells allows late SV40 gene expression without T‐antigen synthesis and DNA replication. These conditions were chosen to analyse the effect of DNA methylation on V‐antigen synthesis detached from the process of DNA replication. We found that in vitro methylation of a single cytosine nucleotide proximal to the major late mRNA cap site by the Hpa II methylase does not block capsid protein synthesis. This result is in contrast to reported data obtained in Xenopus laevis oocyte injection experiments [(1982) Proc. Natl. Acad. Sci. USA 79, 5142‐5146].