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Echinocandin inhibition of 1,3‐β‐D‐glucan synthase from Candida albicans
Author(s) -
Sawistowska-Schröder E.T.,
Kerridge D.,
Perry H.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81032-7
Subject(s) - echinocandin , candida albicans , echinocandins , glucan , enzyme , chemistry , microbiology and biotechnology , glucanase , biochemistry , depolymerization , peptide , polysaccharide , biology , antifungal , organic chemistry , fluconazole , amphotericin b , caspofungin
The cyclic peptide antibiotic echinocandin was found to inhibit 1,3‐β‐D‐glucan synthase activity present in a mixed membrane fraction from Candida albicans . Addition of antibiotic reduced the V max of the enzyme, but the K m was unaffected. GTP stimulated enzyme activity ~ 4‐fold, but did not affect the percentage inhibition of the enzyme by echinocandin. Treatment of the reaction products with α‐amylase and β‐glucanase confirmed that the polymer synthesised was 1,3‐β‐D‐glucan, not glycogen.