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Expression of cloned cDNA for a major surface antigen of Plasmodium falciparum merozoites
Author(s) -
Odink Karel G.,
Lockyer Michael J.,
Nicholls Stephen C.,
Hillman Yvonne,
Freeman Robert R.,
Holder Anthony A.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81027-3
Subject(s) - complementary dna , insert (composites) , plasmodium falciparum , microbiology and biotechnology , biology , plasmid , cdna library , fusion protein , messenger rna , antigen , epitope , expression vector , gene , recombinant dna , genetics , mechanical engineering , malaria , engineering , immunology
A cDNA library of P. falciparum was constructed. Using size‐selected mRNA as a probe several clones were isolated which hybridized to mRNAs larger than 5 kilobases (kb). The cDNA insert of pFC 17, which hybridizes to 5.6‐kb mRNA was expressed by fusion to anthranilate synthetase I in a plasmid expression vector. The expressed fusion protein was shown to contain epitopes of a 195‐kDa protein which is the precursor to 3 major surface antigens of P. falciparum merozoites.