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Basement membrane heparan sulfate proteoglycan from the L2 rat yolk sac carcinoma
Author(s) -
Fenger Mogens,
Wewer Ulla,
Albrechtsen Reidar
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81020-0
Subject(s) - perlecan , heparan sulfate , proteoglycan , basement membrane , chemistry , glucosamine , yolk sac , heparanase , glycosaminoglycan , biochemistry , microbiology and biotechnology , extracellular matrix , biology , embryo
Heparan sulfate proteoglycan from the L2 rat yolk sac carcinoma has been purified and partially characterized. The proteoglycan has an apparent M r of 750000, 35% of which represents the core protein. The core protein seems to be homogeneous, whereas the heparan sulfate chains are heterogeneous with an M r of about 50000–70000, with 30% of the glucosamine being N ‐sulfated. Antibodies raised against the core protein of the heparan sulfate proteoglycan reacted with basement membranes of various rat and human tissue.