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Carbonic anhydrase located on cell surface increases the affinity for inorganic carbon in photosynthesis of Dunaliella tertiolecta
Author(s) -
Aizawa Katsunori,
Miyachi Shigetoh
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81013-3
Subject(s) - carbonic anhydrase , photosynthesis , subtilisin , chemistry , total inorganic carbon , dunaliella , biophysics , mixotroph , algae , biochemistry , botany , carbon dioxide , heterotroph , enzyme , biology , organic chemistry , bacteria , genetics
Low‐CO 2 cells of Dunaliella tertiolecta had higher carbonic anhydrase (CA) activity on their surfaces than inside. The treatment with subtilisin completely abolished CA activity on the cell surface, while the activity inside the cells was not affected by this treatment. Correspondingly, the apparent K m (NaHCO 3 ) for photosynthesis was increased two‐fold, while V max was not affected by this treatment. The apparent K m (NaHCO 3 ) was lowered to the original level when CA was added to the suspension of subtilisin‐treated cells. These results indicate that CA on the cell surface enhances the affinity for inorganic carbon in photosynthesis of D. tertiolecta .