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Mitochondrial compartmentation of metabolic CO 2 resulting from its site of origin in relation to urea synthesis
Author(s) -
Hems Reginald
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80998-9
Subject(s) - urea , chemistry , biochemistry , carbonic anhydrase , glutamate dehydrogenase , mitochondrion , pyruvate dehydrogenase complex , metabolic pathway , metabolic intermediate , metabolism , alanine , acetazolamide , urea cycle , enzyme , glutamate receptor , biology , amino acid , receptor , physiology , arginine
In isolated hepatocytes the entry into urea of metabolic 14 CO 2 ; derived from [ 14 C] formate is modified by the addition of dichloroacetate and hydroxypyruvate. An explanation is that this results from changes in the cytoplasmic/mitochondrial pH gradient. 14 CO 2 , derived from [1‐ 14 C]alanine enters into urea more readily than 14 CO 2 arising from [1‐ 14 C]glutamate. It is proposed that the difference, which is more than 4‐fold, is indicative of a preferred pathway for metabolic CO 2 in liver mitochondria from pyruvate dehydrogenase to carbamoylphosphate synthetase than form oxoglutarate dehydrogenase. Acetazolamide inhibition of carbonic anhydrase is without effect on this observed incorporation into urea.