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Isolation of a calcium‐binding protein from an oxygen‐evolving photosystem II preparation
Author(s) -
Sparrow Raymond W.,
England Reginald R.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80988-6
Subject(s) - photosystem ii , chemistry , calmodulin , calcium , biochemistry , phosphodiesterase , sepharose , affinity chromatography , biophysics , enzyme , biology , photosynthesis , organic chemistry
Heat treatment of a highly active photosystem II preparation (600 μmol O 2 . mg Chl −1 .h −1 ) caused the release of several polypeptides. One of the polypeptides was found to bind to a fluphenazine‐Sepharose affinity column in a calcium‐dependent manner. The purified polypeptide was able to stimulate calmodulindepleted 3',5'‐cyclic nucleotide phosphodiesterase, indicating a possible similarity with calmodulin.